Fig. 9. Quantification of nucleolar localization of the GFP fusion proteins and FU incorporation in nucleoli, in untreated cells and cells treated with ABA. UR61 cells, untreated (Control, upper panel) and treated with 3-aminobenzamide for 8 hours (ABA, lower panel) were transfected with GFP-CTCF, GFP-ZF and GFP. 24 hours post-transfection, cells were pulse-labelled with 5'-FU for 10 minutes and cells showing nucleolar localization of the GFP fusion proteins (bars on the left) and cells showing 5'-FU incorporation in nucleoli (bars on the right) were scored. Data show the percentage of cells (mean ± s.e.m.) from three experiments.