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Figure 1


Fig. 1. Expression of contractile and/or regulatory proteins in primary cultured rat aorta VSMCs. The VSMCs from rat aorta were cultured up to passage ten. (A) Representative western blot images of various proteins in the cell lysates at passages 5-8. (B-F) Protein extracts were run on SDS gels and immunoblotted. For {alpha}-actin, caldesmon (CaD) and calponin (CaP) 2 µg of protein extract was used, all other protein extracts were used at 20 µg. Protein expression levels of (B) total actin, (C) smooth muscle {alpha}-actin isoform, (D) h-calponin, (E) CPI-17 and (F) MYPT1 were measured by immunoblotting and compared with the extract from intact rat aorta, which was normalized to one. n=3-10.