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Figure 1


Fig. 1. Cleavage of human prolactin by chondrocyte lysates at different pHs. (A) Reducing western blot analysis of proteolytic products generated from PRL by incubation of 200 ng of human PRL with 2 µg of protein from rat chondrocyte lysates at various pH values. Arrows indicate 16 kDa and 14 kDa PRL fragments. (B) Effect of the cathepsin-D inhibitor, pepstatin-A on the acidic or neutral proteolytic activity of chondrocyte lysates. Migration of PRL isoforms of various relative molecular mass are shown on the left.