Fig. 6. Mutant CLIP-170 perturbs the focal accumulation of E-cadherin at cell-cell contacts. (A) MCF-7 monolayers were transiently transfected with CLIP-170 -Tail (Tail) or with a control vector expressing GFP alone. After 12-24 hours, cells were fixed and immunolabelled for GFP and E-cadherin to identify the transfected cells and cell-cell contacts, respectively. E-cadherin accumulation was reduced at contacts between cells expressing CLIP-170 -Tail (A, arrow) in comparison with contacts between cells that were untransfected or expressing GFP alone (A, arrowhead). (B) Quantitative image analysis was used to measure E-cadherin accumulation at cell-cell contacts between transfected cells, both expressing either CLIP-170 -Tail or GFP alone (pEGFP). Data are means ± s.e.m. (n=60 contacts) and are representative of three separate experiments.