Fig. 7. Recovery of E-cadherin-YFP fluorescence following photobleaching of cell-cell contacts is perturbed by manoeuvres that disrupt dynamic MTs. Cell-cell contacts between CHO cells stably expressing E-Cad-YFP were photobleached and fluorescence recovery imaged by epi-illumination microscopy as described in the Materials and Methods. (A) Cells were incubated with nocodazole (100 nM) or DMSO (Control). (B) Cells were co-microinjected with HA-tagged CLIP-170 -Tail and mRFP for identification (-Tail) or with mRFP alone (Control). Fluorescence intensity at cell-cell contacts following photobleaching was normalised to the fluorescence intensity at the contacts prior to photobleaching as described in the Materials and Methods.