Fig. 1. Expression of caveolin-1 in HEK293T cells reduced proliferation and altered cell-cycle distribution. HEK293T cells were transfected with the plasmids pEGFP-C1, pEGFP-caveolin-1, pLacIOP or pLacIOP-caveolin-1 and plated 24 hours prior to measurements. Transfection efficiency in these experiments was roughly 50%. (A) Cell proliferation was measured by the MTS® assay. Values shown indicate the percentage of residual activity with respect to non-transfected controls. Black bars show viability of mock controls (pEGFP-C1 or pLacIOP) and hatched bars the viability of caveolin-1-transfected cells (pEGFP-caveolin-1 or pLacIOP-caveolin-1). Values were averaged from three independent experiments (mean ± s.e.m.). ^*Comparison with pEGFP-C1 control (P<0.001); ^#comparison with pLacIOP control (P<0.05). (B) Post-transfection with pEGFP-caveolin-1, the cell-cycle distribution of GFP-positive and GFP-negative HEK293T cells was compared by flow cytometry. Data shown were averaged from three independent experiments (see Table 1).