Fig. 6. Plectin-deficient myocytes display differentiation-dependent changes in AMPK subunit expression. (A) Total cell lysates, prepared from differentiating wild-type (+/+) and plectin-deficient myocytes (-/-) at the days (d) indicated, were subjected to immunoblotting using AMPK 1-, 2- and 1 subunit-specific antisera, as well as antibodies immunoreactive with all AMPK subunit isoforms (total AMPK). Equal amounts of proteins were loaded in each lane and protein bands were visualised as described in the text. The extent of differentiation of plectin^+/+ and ^-/- myoblasts was monitored in parallel using antibodies to caveolin 3, myogenin and MyoD. (B-D) Signal intensities of AMPK subunit bands, densitometrically determined in three independent experiments (including that shown in A), were normalised to total AMPK. Error bars represent the s.e.m. of three independent experiments. Differences between values in wild-type (ple +/+) and plectin-deficient (ple -/-) cells were determined using an unpaired Student's t-test; ^*P<0.05.