Fig. 9. Elevated proportion of Thr172-P AMPK in muscle of newborn plectin^-/- mice. (A) Immunoblot of skeletal muscle (diaphragm) and heart tissue homogenates prepared from plectin^+/+ and plectin^-/- newborn mice using antibodies to unphosphorylated/phosphorylated (total) and phosphorylated (P-) forms of AMPK. (B) Signal intensities of phosphorylated AMPK protein bands were densitometrically determined in three independent experiments (including that shown in A), and normalised to total AMPK. Error bars represent the s.e.m. of these experiments. Differences between plectin^+/+ and plectin^-/- values were determined using an unpaired Student's t-test; ^**P<0.01; P<0.001.