Fig. 4. Quantitative analysis of secretory granule morphology in pancreas. SLOS animals exhibit a 30% decrease in total granule number (A), a 60% decrease in the number of granules with mature morphology (B), and a 55% increase in granules with immature morphology (C). Mature is defined as round vesicles with a membrane bound dense core and no light granular halo. Immature is defined as all other morphologies including `empty' granules. 10 images were analyzed per animal, at 3150X magnification, representative of each area within a section of pancreas. Each group represents the mean ± s.e.m. of four or five animals. ^***P<0.001 compared with counts in the wild-type animals.