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Figure 1


Fig. 1. Adhesion of wild-type and {alpha}2-deficient fibroblasts to extracellular matrix proteins. (A) Equal numbers of wild-type (white columns) and {alpha}2-deficient (black columns) fibroblasts were seeded on triplicate wells coated with optimal concentrations of collagen I (Col I, 40 µg/ml), collagen IV (Col IV, 10 µg/ml), laminin 1 (LM 1, 20 µg/ml) and laminin 5 (LM 5, 5 µg/ml) as indicated. After 30 minutes, adhesion of {alpha}2-deficient fibroblasts to collagen I is slightly lower than that of wild-type cells. The mean average of triplicate wells and s.d. are shown. (B,C) At the end of the adhesion assay, wild type (+/+) and integrin {alpha}2-deficient (-/-) fibroblasts adhering to collagen I were photographed under phase-contrast microscopy.