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Figure 5


Fig. 5. The {alpha}2ß1 integrin is indispensable for keratinocyte adhesion to collagens. (A-C) Lysates of primary keratinocytes (K) and fibroblasts (F) from wild type (+/+) and {alpha}2-deficient (-/-) mice were immunoblotted with primary antibodies against the integrin {alpha}1 (A), {alpha}2 (B) and {alpha}11 (C) subunit as indicated. (D) Equal numbers of wild-type (white columns) and {alpha}2-deficient (black columns) keratinocytes were seeded on triplicate wells coated with collagen I (Col I), collagen IV (Col IV), laminin 1 (LM 1) and laminin 5 (LM 5) as described in Fig. 1A. Compared with wild-type cells, there is nearly no adhesion of {alpha}2-deficient keratinocytes to collagen I and collagen IV whereas adhesion of both strains of keratinocytes to laminin 1 and laminin 5 is similar. (E) Wild-type mouse keratinocytes were seeded on coats of collagen I (Col I), collagen (IV) and laminin 1 (LM 1) in the absence (- ab) or presence (+ ab) of monoclonal antibody against integrin {alpha}2 subunit. The antibody inhibits keratinocyte adhesion to collagens but not laminin 1.