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Figure 6


Fig. 6. Cbfa-1 and MMP-13 expression are reduced in iNOS-deficient embryos. Immunohistological detection of Cbfa-1 (A,C,E,G,I,K,M,O) and MMP-13 (B,D,F,H,J,L,N,P) on sections from iNOS wild-type (WT) (left side) and iNOS-deficient (right side) 17-day-old embryos. Experiments were performed in triplicate on sections from three different embryos. Skulls (E,F,GH), ribs (I,J,K,L) and vertebrae (M,N,O,P) are magnified. m, Meckel's cartilage; mb, maxillary bone; nb, nasal bone; hbc, cartilage of the hyoid bone; cc, cricoid cartilage. (Q) Immunoblot detecting the levels of Cbfa-1, MMP-13 and GAPDH from WT (+/+) and iNOS-deficient (NOS2, -/-) mouse embryo lysates (n=3).