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Figure 5


Fig. 5. Identification of {alpha}ENaC in human endothelial cells (HUVEC; representative experiment). Membrane proteins were separated by SDS-PAGE and stained with Coomassie brilliant blue (lane 1). {alpha}ENaC was detected by using an anti-{alpha}ENaC antibody (lanes 2-4). The amount of {alpha}ENaC in the aldosterone-treated sample (A) is about twice that in the control (C) and about 6 times higher than that of aldosterone and spironolactone (A+S)-incubated HUVEC. As positive control (PC), we used ENaC-expressing oocytes. The molecular mass standard is given on the left. The upper band in the range of 90 kDa most likely represents a glycosylated form of ENaC.