Fig. 5. Arsenite induces the accumulation of FMRP in stress granules. (A) HeLa cells grown in the absence of treatment (naive), the presence of 20 µM AdOx for 24 hours, the presence of 0.5 mM sodium arsenite for 30 minutes, or pre-treated with 20 µM AdOx for 24 hours and then treated with 0.5 mM sodium arsenite for 30 minutes were immunostained with anti-PABP pAb (green) and anti-FMRP mAb (red) as in Fig. 3. (B) FMRP small cytoplasmic granules lack asymmetric dimethyl-arginine. HeLa cells treated as in A were immunostained with anti-mRG pAb (green) and anti-FMRP mAb (red).