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Figure 5


Fig. 5. Targeting of L-plastin phosphorylation variants to surface-F-actin spikes in epithelial LLCPK1 cells. LLCPK1 cells expressing Ser5Glu (S/E, upper panels), Ser5Ala (S/A, middle panels) or WT L-plastin (WT, lower panels) were double-stained with Rhodamine-phalloidin (left panels) and anti-sv-tag antibody (right panels). Cy2-coupled anti-rabbit IgG served as secondary antibody. Bars, 15 µm.