Fig. 4. Arabidopsis peroxisomes bearing overexpressed myc-AtPex11c and -d became elongated/tubulated (2.5-72 hours), whereas peroxisomes acquiring myc-AtPex11b became spherical. (A-H) A representative single transformed cell in each panel illustrates elongated peroxisomes possessing myc-AtPex11c (A,C,D) or myc-AtPex11d (E,G,H). Arrows point to examples of perfect colocalization between these myc-tagged proteins (A,E) and endogenous peroxisomal catalase (B,F). Arrowheads point to normal spherical or rod-shaped peroxisomes in neighboring nontransformed cells. (I-L) Single representative transformed cells show spherical/toric peroxisomes bearing overexpressed myc-AtPex11b (I,K,L) perfectly colocalized with endogenous peroxisomal catalase (compare I and J). (M-P) Peroxisomes bearing CAT-SKL (M,O,P) are spherical or rod-shaped and are perfectly colocalized with endogenous catalase (compare M and N). (Q, lower panel) Graphical representation of the percentage of transformed cells bearing peroxisomes 3 µm at various time points post-bombardment (n30 for all data points). Cells were bombarded with myc-AtPEX11a (), -b(), -c (), -d (), -e () or CAT-SKL (), fixed at the indicated time points (x-axis), and immunolabeled with anti-myc plus Cy-2-conjugated antibodies or anti-CAT plus Cy-2-conjugated antibodies. Intercepts at the y-axis (0 hours) were projected to the baseline (3% elongation) for each construct. Bar, 10 µm.