JCS -- Monier et al. 120 (1): 101 Figure IG2

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Fig. 2. H3S10P foci are enriched in late S-phase cells and correlate with cyclin B1 expression. (A) NT2 cells submitted to a short BrdU pulse were counterstained with DAPI (grey) and labelled with BrdU (green) and H3S10P (red). Merged images illustrate the four classes of nuclei analyzed: BrdU^-/H3S10P^- (c), BrdU⁺/H3S10P^- (f), BrdU⁺/H3S10P⁺ (i) and BrdU^-/H3S10P⁺ (l). The percentage of nuclei falling into each category is indicated on the right, as well as the number of H3S10P foci (mean ± s.d.). (B) NT2 cells were counterstained with DAPI (grey) and labelled with cyclin B1 (Cyc B1, green) and H3S10P (red). Merged images illustrate the four classes of nuclei analyzed: Cyc B1^-/H3S10P^- (c), Cyc B1⁺/H3S10P^- (f), Cyc B1^-/H3S10P⁺ (i), Cyc B1⁺/H3S10P⁺ (l). The percentage of nuclei falling into each category is indicated on the right. Bars, 10 µm.