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Figure 5


Fig. 5. Heterochromatic nature of large pericentromeres in nuclei exhibiting no more than ten H3S10P or Aur-B foci. (A-B) NT2 nuclei immunodetected with H3S10P (yellow) and 5-meC (red in A) or HP1{alpha} (red in B), exhibiting fewer than ten (a-d) or more than ten H3S10P foci (e-h). (C) Histogram of the total number of 5-meC accumulations, positive (red) or negative (yellow) for H3S10P according to the number of foci per NT2 nucleus, as displayed in A. (D) Histogram of the percentage of 94 randomly taken NT2 cells exhibiting HP1{alpha} accumulations, as shown in B, according to the presence of pericentromeric H3S10P foci (no foci, fewer than ten foci, at least ten foci). (E,F) H3K9me (red in E) or H3K9me3-S10P (red in F) foci colocalize with Aur-B (yellow) in NT2 nuclei exhibiting fewer than ten (a-d) or more than ten nuclear foci (e-h). (G,H) H3S10P-A594 foci (red) colocalize with H3S10P (yellow in G) and H3K9me3S10P (yellow in H) in NT2 nuclei exhibiting fewer than ten (a-d) or more than ten nuclear foci (e-h). Bars, 10 µm.