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Figure 5


Fig. 5. Ectopic Ciz1 is immobilized through C-terminal interactions. (A) Ectopically expressed GFP-ECiz1 (green) in unextracted nuclei, and after extraction with Triton X-100, or Triton X-100 followed by DNase 1 and 0.5 M NaCl. (B) Ectopically expressed GFP-C275 after the same treatments and (C) GFP-ECiz1 N471 in untreated nuclei and after extraction with Triton X-100. DNA is stained with Hoechst 33258 (blue) and imaged to reflect the DNA content after each treatment.