Fig. 6. Paxillin phosphorylation is regulated by mechanical force. Treatment with an actomyosin contractility inhibitor (H7) leads to disassembly of FAs and formation of FXs, as seen in the frames from a time-lapse movie of PAECs expressing YFP-paxillin (top). Preceding the disassembly there is an increase in paxillin phosphorylation levels, as indicated by immunolabeling of cells for paxillin and phosphopaxillin after 1 minute of treatment with H7 (bottom). Single labels are spectrum scaled for intensity, using the same scale for control and treated cells. The ratio image was scaled according to the provided look-up table. Bar, 5 µm.