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Figure 8


Fig. 8. FAK mediates the effect of paxillin phosphorylation on adhesion dynamics. Paxillin phosphorylation mutants do not have any effect on adhesion dynamics when expressed in FAK-knockout mouse embryonic fibroblasts (MEFs), whereas they do affect the dynamics in FAK-knockout cells re-expressing FAK (A). Displayed are smoothing spline fits ({lambda}=100) based on autocorrelation analysis on nine movies for each condition. When FAK is expressed along with wild-type paxillin in PAECs, it substantially increases adhesion dynamics (B). Expression of Y2E-paxillin or Y2F-paxillin along with FAK does not modify the dynamics any further. The time constant for the correlation decay ({tau}) is given in parentheses, along with the R2 of the fitting spline fit.