Fig. 10. Silencing of act9 affects the pumping cycle of the contractile vacuoles in misshapen transformants. While the osmoregulatory complex works normally in act9-silenced cells with normal shape, its pumping cycle is strongly prolonged in triangle-shaped cells. Feeding with pPD vector does not cause any defect in the cycle of the contractile vacuole complex. Data were collected in three different experiments from at least ten cells per group. wt, wildtype. Error bars represent s.e.m.