Fig. 2. GFP localization of members of the act1 subfamily. (A-C) A confocal time series of a cell transformed with GFP-act1-2 reveals actin comet tails that propel vacuoles (two labeled ^* and +) through the cytoplasm. (D-F) Confocal z series of a cell transformed with GFP-act1-2. Note the irregular distribution of patches around the surface of some food vacuoles (fv). (G,H) Transformation with GFP-act1-6 resulted in diffuse cytosolic staining without any specific localization (G, median; H, superficial plane). (I-N) In cells transformed with GFP-act1-9, actin comet tails on food vacuoles occurred. Food vacuoles can move in opposite directions through the cytoplasm (see the movement of the two vacuoles labeled with ^* and + within 150 seconds). See supplementary material Movie 1 for the thin, very dynamic tails that can propel some food vacuoles and small vesicles through the cytoplasm and actin filaments that are catapulted into the cytoplasm from the posterior end of the oral cavity. mn, macronucleus; oc, oral cavity; fv, food vacuoles. Bars, 10 µm.