Fig. 6. FasL is monoubiquitylated in vivo. Western blots of cell lysates from stable transfectants of RBL expressing equivalent levels of WT or FasL-GFP mutants (KKR, -Pro and 3Y) or 1/10 of the immunoprecipitates both probed with anti-FasL, G247-4 and 9/10 of the same immunoprecipitate probed with anti-Ub, P4D1. Arrows denote the position of bands corresponding to ubiquitylated FasL (Ub-FasL-GFP) and IgG heavy chain (IgGHc). Note that both KKR and -Pro constructs lack K72 and K73 (Fig. 1). Positions of molecular masses are shown (kDa).