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Figure 5


Fig. 5. Purification of sperm FUT. (A) Representative Mono-Q chromatogram of sperm extracts. The protein fractions (1-10) were desalted and further purified using a GDP-agarose column. They were then subjected to 12% SDS-PAGE analysis with silver staining (B) and western blot analysis using the anti-FUT5 antibody N-18 (B).