Fig. 2. Knock-down of SNX5 and SNX6 as shown by RT-PCR. Transfection of HeLa cells with individual SNX5 siRNA, SNX6 siRNA or respective SMARTpools led to a strong reduction of RNA levels as shown by RT-PCR. (-) control sample without template; (contr.) indicates template from the control cells treated with a scrambled siRNA; (D1, D2, D3, D4) individual siRNAs for SNX5 or SNX 6; (Pool) SMARTpool. For all further experiments in this study, we selected SNX5 D1 and SNX6 D2 siRNA, although similar results were obtained with the other siRNAs or the pools (see supplementary material Fig. S2). GAPDH-specific PCR primers were used as control amplicon.