Fig. 5. RNAi-mediated suppression of SNX5 and/or SNX6 perturbs the kinetics of retromer-mediated endosome-to-TGN transport of CD8-CI-MPR. (A) Images depicting the kinetics of delivery of cell-surface-labelled CD8-CI-MPR (green) to the TGN46-labelled TGN (red) in control or SNX5- and/or SNX6-suppressed HeLaM cells. Images are projections of eight confocal sections and are representative of >50 cells imaged for each condition. Bar, 20 µm. (B) Quantification of the kinetics of cell-surface-labelled CD8-CI-MPR transport to the TGN46-labelled TGN in HeLaM cells expressed as percent colocalization between the two labels, normalised to control cells. Data were obtained as outlined in Materials and Methods from n>50 cells for each time point, and are expressed as the mean ± s.d.