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Figure 2


Fig. 2. (A) {gamma}-H2AX-containing S-foci partially colocalize with RAD51 foci on SCs. (a) Partial mid-pachytene chromosome spread stained with anti-{gamma}-H2AX (green), anti-RAD51 (red) and anti-SCP3 (blue) antibodies. Examples of non-colocalized {gamma}-H2AX and RAD51 foci are indicated by white arrowheads. Mixed {gamma}-H2AX/RAD51 foci are marked by red arrows. (b-c) Higher magnification of one synapsed chromosome core (b) triple-labeled with anti-{gamma}-H2AX (green), anti-RAD51 (red) and anti-SCP3 (blue) antibodies, (c) double-labeled with anti-{gamma}-H2AX, and anti-RAD51 are shown. {gamma}-H2AX and RAD51 foci are slightly offset to show their colocalization more clearly (d). Red and white arrows indicate sites of colocalized and juxtaposed foci, respectively. (B) {gamma}-H2AX S-foci partially colocalize with MLH1 foci on SCs. (a) Partial pachytene chromosome spread stained with anti-{gamma}-H2AX (green), anti-MLH1 (red) and anti-SCP3 (blue) antibodies. Examples of non-colocalized {gamma}-H2AX and MLH1 foci are indicated by white arrowheads. The only mixed focus on this image is indicated by a red arrow. (b-d) Enlarged images of one SC triple-labeled with (b) anti-{gamma}-H2AX (green), anti-MLH1 (red) and anti-SCP3 (blue) antibodies, and (c) double-labeled with anti-{gamma}-H2AX and anti-MLH1 antibodies. (d) {gamma}-H2AX and MLH1 foci are slightly offset. White arrows indicate juxtaposed foci; red arrows indicate colocalized foci. Bars, 10 µm.