Fig. 8. The R30D mutant cannot rescue depletion phenotypes of coronin 1B. (A) Rat2 fibroblasts were infected with lentivirus expressing coronin 1B shRNA (KD-1B) without or with GFP-tagged wild-type or GFP-tagged R30D coronin 1B or with control shRNA (NS). Cells were subjected to single-cell tracking. Data are presented as mean cell speed, error bars indicate 95% confidence intervals. (N, number of cells tracked per treatment; ^**P<0.01). (B-D) Cells described in A were subjected to kymography analysis. Protrusion parameters were determined from at least six randomly selected cells; n, number of protrusion events analyzed per treatment. Data are presented as the mean, error bars indicate 95% confidence intervals. Dunnett multiple comparison test was performed as a post-test for one-way ANOVA using the first column as control; ^*P<0.05, ^**P<0.01.