Fig. 2. TGFβ1 induces regression of CLS and apoptosis of BREC. (A) Phase-contrast image of BREC after treatment with VEGF plus FGF2 for 2 days, followed by VEGF, FGF2 plus TGFβ1 for an additional 2 days. (B) Quantification of the effects of TGFβ1 on the length of CLS and number of BrdU-positive cells. A pre-established capillary network completely regressed after addition of TGFβ1 for 2 days (^*P<0.0001 vs VEGF plus FGF2) and proliferation was significantly decreased within the 13 hours of the assay (^#P<0.002 vs VEGF plus FGF2). (C) Images of the same field of CLS assayed for apoptosis and proliferation after treatment with VEGF (25 ng/ml), FGF2 (2.5 ng/ml) plus TGFβ1 (1 ng/ml). Bar, 100 µm.