Fig. 8. SNX1 modulates E-cadherin recycling. (A) SNX1-depleted cells and scramble-siRNA-treated cells (control) were fixed and stained for SNX1 (red), E-cadherin (green) and nuclei (DAPI, blue) at steady-state (a,d), after chelation of extracellular Ca²⁺ (b,e) or following restoration of Ca²⁺ levels for 60 minutes (c,f). Notice the retarded reformation of cell-cell junctions and the intracellular accumulation of E-cadherin (arrows) in SNX1-depleted cells. Insets depict higher magnification images of boxed regions. (B) Surface proteins in SNX1-depleted cell monolayers or scramble-siRNA-treated cell monolayers were biotinylated before Ca²⁺ chelation (Surface, Sur.), after chelation (EDTA) and after restoration of Ca²⁺ levels for 60 minutes (+Ca2+), and levels of surface E-cadherin, EGFR and TfnR were analyzed. Bar, 20 µm.