Fig. 2. H2B_T119ph on the XY body and on meiotic and mitotic metaphase chromosomes. (A) Double immunostaining of pachytene and diplotene spermatocyte nuclei with anti-SYCP3 (red) and anti-H2B_T119ph (green). The insert shows a larger magnification of the area containing the XY body. Arrowheads, pseudoautosomal synapsed region; arrows, patches of synaptonemal complex lateral elements that are H2B_T119ph negative. (B) H2B_T119ph (green) and SYCP3 (red) on meiotic metaphase (left) and H2B_T119ph (green) on DAPI-stained (blue) mitotic HeLa cell nucleus (right). Bars, 10 µm.