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Figure 7


Fig. 7. Derepression of X chromosomal genes in Hr6b-knockout spermatids. Real-time RT-PCR quantification of mRNA levels of four autosomal genes (A) and six X chromosomal genes (B) in a mixed spermatocyte/spermatid (spc/spt) and purified spermatid (spt) cell preparation from wild-type (wt) and Hr6b-knockout (ko) mice. The amount of PCR products was normalized to Actb (beta-actin) mRNA (x100). Results from two independent experiments are shown. For each experiment, the value represents the average of a duplicate real-time PCR experiment.