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Figure 2


Fig. 2. Inhibition of neurite extension by TTC3 overexpression. PC12 cells were transfected with GFP-empty, GFP-TTC3 and GFP-TTC3 mutants. The cells were treated with NGF 12 hours after transfection, allowed to differentiate for 3 days and analyzed by immunofluorescence microscopy. (A) Cells transfected with TTC3 showed a dramatic reduction of neurite sprouting, when compared to control cells. PHD, phalloidin; HOE, Hoechst 33258. Bar, 10 µm. (B) The percentage of differentiated cells, defined as those bearing at least one neurite longer than the main cell body axis, was quantitatively analyzed in the indicated transfections. The histograms represent the average of three independent experiments. All the differences were statistically significant (P<0.001, {chi}-square test), except the one detected between the {Delta}Cterm and {Delta}CBR mutants. Error bars represent s.d.