Fig. 5. Rho kinase inhibition is unable to revert the TTC3 overexpression phenotype in differentiated PC12 cells. (A) PC12 cells were transfected with an empty GFP or GFP-TTC3 construct, treated with NGF after 12 hours and allowed to differentiate for 2 days. Afterwards, the culture medium was changed and vehicle alone (MOCK) or Y27632 were added. The cells were kept in culture for additional 18 hours and then processed for immunofluorescence with anti GFP antibodies (green) and phalloidin (red). Bar, 10 µm. (B) Quantitative analysis of the percentage of differentiated cells (left panel) and of neurite length (right panel) in the above experiments, performed as in Figs 2 and 3, respectively.