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Figure 4


Fig. 4. The effect of C3 exotoxin on ciliogenesis. (A) Confocal photomicrographs of actin stained with phalloidin (red) and of {gamma}-tubulin (green) in mTEC cultures on ALI day 3 and ALI day 5 following treatment with vehicle or C3 exotoxin initiated on ALI day 2 and day 4, respectively. Reconstructed z-axis images are below. (B) The percentage of apical {gamma}-tubulin in all cells with basal bodies (apical or cytoplasmic) and apical beta-tubulin in total cells treated as in A. Data are the mean ± s.d. (n=3 experiments); *P<0.05, significance compared with vehicle. (C) Basolateral beta-catenin (red) and DAPI-stained cell nuclei (blue) in ALI day 3 cultures following treatment with vehicle or C3 exotoxin at ALI day 2. Bars, 10 µm.