Fig. 2. Nogo-66 enhances the adhesion of OECs. (A) Photomicrographs of OECs plated for 3 hours, 6 hours and 12 hours on spots of 100 µg/ml GST, GST–Nogo-66, His or His–Nogo-66, respectively. (B) Quantitative assessment of the number of cells attaching to the protein spots at various time points. Values are reported as mean ± s.d./visual field. ^*P<0.05, ^**P<0.01 versus vehicle. (C) Enhancement of adhesion of OECs by Nogo-66 was dose-dependent. After 6 hours, OECs attached to GST, GST–Nogo-66, His or His–Nogo-66 spotted for a series of concentrations (µg/ml) were quantitatively assessed.