Fig. 4. The effects of Nogo-66 on the migration and adhesion of OECs are mediated by NgR. (A) OECs were pre-treated with or without PI-PLC (0.1 U/ml), NgR antibody (1:100) or goat IgG (1:100) for 2 hours and applied to Boyden chamber assays. The trans-well membranes were coated with laminin, GST, GST–Nogo-66, His or His–Nogo-66 (100 µg/ml each). SCs were served as a negative control. (B) After pre-treatment with or without either PI-PLC, NgR antibody or goat IgG, OECs or SCs were plated for 6 hours on spots of GST, GST–Nogo-66, His or His–Nogo-66. Quantitative assessment was obtained by determining the percentage of the cell numbers within the spots compared with outside the spots (vehicle, 100%). (C) Dose-dependency of the effect of PI-PLC. After being exposed to PI-PLC at the indicated concentration for 2 hours, cells were harvested and used in an adhesion assay. Values were reported as mean ± s.d./visual field. ^**P<0.01 versus laminin or vehicle.