Fig. 1. P-cofilin levels change in response to alterations in actin filament organisation and PI3K signalling. (A) Representative 2D-DIGE gel images and 3D-fluorescence profiles of acidic and basic cofilin isoforms are shown for untreated (Ctrl) cells and cells treated with control dsRNA and dsRNA targeting cofilin, PTEN, PI3K p110 catalytic subunit (p110), Cdc42, Rac1 and Rac2 (Rac1/2), and Arp3. (B) The relative abundances of P-cofilin (acidic isoform) and cofilin (basic isoform) are shown for RNAi-treated cells from 2D-DIGE image analysis. Values represent the average of three measurements from biological replicates. Error bars represent the standard deviation. (C) Immunoblots show P-cofilin levels in untreated control and dsRNA-treated S2R⁺ cell lysates confirming 2D-DIGE data. Relative abundance is shown below the blot as a percentage of the control, and is the average calculated from densitometry measurements of blots from three independent experiments. (D) Immunoblot of P-Akt and total Akt levels in control and dsRNA-treated S2R⁺ cell lysates confirms the loss of PI3K, PTEN and Akt expression.