Fig. 5. Vn binds PrP^C in vivo. (a) Confocal images of dissociated DRG cells treated with Alexa 568-Vn (red) and immunolabeled with anti-PrP^C (green) are shown in the top row. Confocal images of SN56 cells transfected with GFP-PrP^C (green) and treated with Alexa 568-Vn (red) are shown in the bottom row. (b) Pull down assay from cell extracts incubated with Vn-Sepharose. Western blots of Vn-Sepharose-bound proteins from untransfected (lanes 1 and 4), GFP transfected (lanes 2 and 5) or GFP-PrP^C transfected (lanes 3 and 6) HEK293 cells. Blots immunolabeled with anti-GFP (lanes 1 to 3) or anti-PrP^C (lanes 4 to 6) antibodies revealed that PrP^C, but not GFP alone, binds to Vn.