Fig. 4. Wrch-1 regulates cell migration. (A) Wrch-1 depletion inhibits HelaS3 cell migration. Cells were transfected with the indicated siRNAs for 24 hours and subsequently re-plated into a 12-well plate to obtain a confluent layer in 24 hours. Monolayer wound healing assays were performed as described in Materials and Methods. Migration was quantified 24 hours after wounding. Shown is the average ± s.e.m. made over ten to 12 sites. Data shown are representative of three independent experiments. (B) Wounding-induced Wrch-1 mRNA induction was performed as described in Materials and Methods. Shown are the mean and s.e.m. of three independent experiments. Some s.e.m. are smaller than the size of the data points.