Fig. 6. Loss of BAF causes an increase in the number of cells present in the early stages of S phase. HeLa-S3 cells were treated with luciferase RNAi (control RNAi; black bar) or BAF RNAi (BAF RNAi; white bar) using Lipofectamine PLUS and labeled with BrdU. These BrdU-positive cells were classified into five types (I, II, III, IV and V) based on patterns of replication foci as described in O'Keefe et al. (O'Keefe et al., 1992): I for early stage, II for middle, III middle-late, IV for late, and V for late: typical examples are shown in the top panels. The proportion of BrdU-positive cells in each stage was presented as a percentage. Approximately 200 cells were counted for each of 13 independent experiments. The means of the 13 experiments for cells treated with luciferase RNAi (control RNAi) or BAF RNAi (BAF RNAi) are shown. Error bars represent s.d.