Fig. 8. Loss of BAF causes disassembly of emerin and lamin A from the nuclear envelope. (A) Quantitative western blotting using the antibodies indicated on the left. 25 µg (lane 1, extract corresponding to 5x10⁴ cells), 12.5 µg (lane 1/2), 5 µg (lane 1/5), 2.5 µg (lane 1/10) of whole cell extract from cells treated with luciferase RNAi (control RNAi, left) or BAF RNAi (right) using Oligofectamine. Upper and lower bands in lamin A/C are lamin A and lamin C, respectively. (B) Indirect immunofluorescence staining using anti-BAF, anti-emerin, anti-lamin A, and anti-lamin B antibodies of cells treated with luciferase RNAi (control RNAi; left panels) or BAF RNAi (BAF RNAi; right panels). Chromosomes were stained with DAPI. Bar, 20 µm.