Fig. 1. Pdd1p shows dynamic localization during development. C-terminal CFP (A-D) and N-terminal GFP (E-H) Pdd1p fusion protein (constructs shown) localization was visualized by fluorescence microscopy of live cells, co-stained with 4',6-Diamidino-2-phenylindole (DAPI). (DAPI accumulation in vacuoles can produce significant cytoplasmic fluorescence in addition to nuclear staining.) Differential interference contrast images (DIC) of cells are paired with GFP or CFP and DAPI fluorescence. The observed nuclear Pdd1p localization is illustrated in the chronological progression through conjugation in the top diagram as gray shading. Image panels corresponding to the illustrations are: (A) prophase Meiosis I; (B) metaphase Meiosis I; (C) nuclear exchange (data not shown for post-zygotic divisions and new macronuclei emergence – Mac I); (D,E) early macronuclear differentiation (Mac II); (F-H) macronuclear development after pair separation (exconjugants Mac II). Selected nuclei are labeled in the illustration and on the DIC images as: om, old macronuclei; mic, micronuclei; gmi, gametic micronuclei; relic, discarded meiotic products; zmi, zygotic micronuclei; NM, new macronuclear anlagen.