Fig. 1. Intracellular distribution of Chs4p and Chs3p. (A,B) Chs4p-GFP and Chs3p-GFP were localized by fluorescence (A) and confocal microscopy (B) in CRM233 and CRM103 strains, respectively. (C) Subcellular fractionation in continuous sucrose gradients of cell extracts obtained from the strain JAY29 containing plasmid pRS314-CHS4-GFP. Protein amounts were determined in even fractions by western blot using specific antibodies. The graph shows the quantitative analysis of these western blots data.