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Figure 2


Fig. 2. Intracellular distribution of Chs4p-GFP. (A) Localization of Chs4p-GFP in the indicated strains. Note the accumulation at the septum but also the defined staining of the cell periphery in mother and daughter cells. (B) Intracellular accumulation of Chs4p-GFP in the indicated strains after transfer of the cells to galactose. Arrowheads indicate Chs4p deposition along bud membrane. Numbers indicate the time (in minutes) after induction. Wt and chs3{Delta} were transformed with the pRS314-GAL1-CHS4-GFP plasmid. All the strains used in the figure are W303 derivatives.