Fig. 4. Intracellular distribution of Chs3p in the chs4 mutant. (A) Localization of Chs3p-GFP in the indicated strains. Arrowheads indicate Chs3p-GFP localization at the neck in the chs4 strain. Note the reduced intensity of the label compared with the wild type. (B) immunofluorescence localization of Chs3p-3xHA in wild-type, chs4 and chs5 strains (Y1306 background). Note the high definition of staining in the wild type in contrast to the diffuse and dotted pattern in the chs4. Chs3p was retained prominently in Golgi vesicles in the chs5. Data from wild type and chs4 are also expressed in graph format as percentages (n=230/240) of cells with the indicated distribution pattern, referred to the number of cells with defined staining. Discrete distribution refers to cells presenting staining sharply defined at the plasma membrane; Diffuse, polarized staining not sharply defined; Bud, cells showing diffuse staining at bud periphery.