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Figure 5


Fig. 5. Immunodetection of Chs3p-HA and markers Pma1p and Pep12p in discontinuous sucrose gradients using cell extracts from CRM872 (chs3{Delta}) and CRM278 (chs3{Delta} chs4{Delta}) strains transformed with the pHV7-C-HA. Western blots and their quantitative analyses are shown. Data are from a single representative experiment, but average values for several experiments are indicated in the text.