Fig. 9. Chs4p distribution in the absence of endocytosis. Calcofluor staining and Chs4p distribution in the end4 chs4 mutant transformed with pRS315 plasmids containing either the CHS4-GFP or CHS4590-GFP constructs. Chs4p-GFP distribution did not change as a result of the end4 mutation, independently of the C-terminal region of the protein (compare with Fig. 3A). Yeast cells were grown at 25°C.