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Figure 1


Fig. 1. (A-C) Clathrin, AP1 and retromer localization on STxB-containing EE/RE. STxB-HRP was bound to HeLa cells kept on ice that were then incubated for 45 minutes at 19.5°C. Cells were prepared for whole-mount analysis, followed by immunogold-labeling with antibodies against (A,B) clathrin or (C) the AP1 subunit {gamma}-adaptin (10-nm gold particles), and in all cases to Vps26 (15-nm gold particles). STxB-HRP-containing membranes appeared electron-dense owing to the polymerization product. Note that some structures are double-labeled for clathrin-AP1 and Vps26 (arrows), whereas others are stained for clathrin or {gamma}-adaptin only (arrowheads). Bars, 200 nm.